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Improved expression, purification, and crystallization of p38α MAP kinase

✍ Scribed by Marina Bukhtiyarova; Katrina Northrop; Xiaomei Chai; David Casper; Michael Karpusas; Eric Springman

Book ID
116827895
Publisher
Elsevier Science
Year
2004
Tongue
English
Weight
446 KB
Volume
37
Category
Article
ISSN
1046-5928

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## Abstract The recombinant human p38 MAP kinase has been expressed and purified from both __Escherichia coli__ and SF9 cells, and has been crystallized in two forms by the hanging drop vapor diffusion method using PEG as precipitant. Both crystal forms belong to space group __P__2~1~2~1~2~1~. The