A simple inexpensive sensitive protease assay was developed using soluble fluorescein isothiocyanate (FITC)-labeled casein. Casein was reacted with FITC to form the fluorescein thiocarbamoyl derivative. This substrate is cleaved by trypsin, chymotrypsin, elastase, subtilisin, and thermolysin in a li
Improved Calpain Assay Using Fluorescein Isothiocyanate-Labeled Casein
β Scribed by S.M. LONERGAN; M.H. JOHNSON; C.R. CALKINS
- Book ID
- 108820608
- Publisher
- Institute of Food Technologists
- Year
- 1995
- Tongue
- English
- Weight
- 261 KB
- Volume
- 60
- Category
- Article
- ISSN
- 0022-1147
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This report describes an assay for measuring the activity of collagenases using reconstituted fibrils of fluorescein-labeled soluble collagen as substrate. The labeling of commercially available material is easy and not expensive. The assay is very sensitive, reproducible, and is linear with collage