𝔖 Bobbio Scriptorium
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A handy assay for collagenase using reconstituted fluorescein-labeled collagen fibrils

✍ Scribed by Antonio Baici; Gabriel Cohen; Kurt Fehr; Albert Böni

Publisher
Elsevier Science
Year
1980
Tongue
English
Weight
224 KB
Volume
108
Category
Article
ISSN
0003-2697

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✦ Synopsis

This report describes an assay for measuring the activity of collagenases using reconstituted fibrils of fluorescein-labeled soluble collagen as substrate. The labeling of commercially available material is easy and not expensive. The assay is very sensitive, reproducible, and is linear with collagenase concentration up to 100% consumption of the reconstituted fib&. Readings of collagenolytic activity can be determined directly by measuring the fluorescence of the released labeled peptides without further processing of the data.

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A fluorescent screening assay for collagenase using collagen labeled with 2-methoxy-2,4-diphenyl-3(2H)-furanone
✍ Robert L. O'Grady; Andrew Nethery; Neil Hunter 📂 Article 📅 1984 🏛 Elsevier Science 🌐 English ⚖ 449 KB

This report describes the use of the compound 2-methoxy-2,4-diphenyl-3(2H)-furanone to label collagen as a substrate for the detection of mammalian collagenase in a fluorescent assay which is suitable for screening large numbers of samples. The compound 2-methoxy-2,4-diphenyl-3(2H)-furanone presents