✦ LIBER ✦

Improved automated simultaneous determination of isosorbide dinitrate and its metabolites in plasma by capillary column gas chromatography

✍ Scribed by Lutz, D. ;Rasper, J. ;Gielsdorf, W. ;Settlage, J. A. ;Jaeger, H.

Publisher: John Wiley and Sons
Year: 1984
Tongue: English
Weight: 550 KB
Volume: 7
Category: Article
ISSN: 0935-6304
DOI: 10.1002/jhrc.1240070202

No coin nor oath required. For personal study only.

✦ Synopsis

An automated specific and sensitive method for the simultaneous determination of isosorbide dinitrate and its metabolites isosorbide-2-mononitrate, isosorbide-5-mononitrate, isomannide mononitrate, and isoidide mononitrate at concentrations down to 0.5,1,3,2, and 2 nglml, respectively, in human plasma is described. The procedure involves the extraction of the drug and its metabolites together with their internal standards isomannide dinitrate and o-nitrobenzyl alcohol from plasma with dichlorornethane.

The analysis iscarried out by fused silica capillary gas chrornatography using a 63Ni-electron capture de1,ector. The method is applied to the pharmacokinetics of isosorbide dinitrate in humans.

📜 SIMILAR VOLUMES

Simultaneous determination of isosorbide

Simultaneous determination of isosorbide dinitrate and its mononitrates in human plasma by capillary column GLC

✍ M. T. Rosseel; M. G. Bogaert 📂 Article 📅 1979 🏛 John Wiley and Sons 🌐 English ⚖ 223 KB

0 A previously described electron-capture CLC method for determination of isosorbide dinitrate in human plasma was adapted for the simultaneous determination of isosorbide dinitrate, isosorbide 2mononitrate, and isosorbide 5-mononitrate using a capillary column. Quantitation was done with two intern

Assay of glyceryl trinitrate, isosorbide

Assay of glyceryl trinitrate, isosorbide dinitrate, and their metabolites in plasma by large-bore capillary column gas-liquid chromatography

✍ Brian P. Booth; Brian M. Bennett; James F. Brien; Douglas A. Elliott; Gerald S. 📂 Article 📅 1990 🏛 John Wiley and Sons 🌐 English ⚖ 636 KB

Two large-bore capillary columns, one with dimethyl polysiloxane (HPl@) as the stationary phase and the other with phenyl (50 per cent) methyl (50 per cent) polysiloxane (DB-17), were used to develop gas-liquid chromatographic (GLC) assays for measuring isosorbide dinitrate (ISDN), glyceryl trinitra

Determination of isosorbide 5-mononitrat

Determination of isosorbide 5-mononitrate in human plasma by capillary column gas chromatography

✍ P. Straehl; R. L. Galeazzi 📂 Article 📅 1984 🏛 John Wiley and Sons 🌐 English ⚖ 322 KB 👁 2 views

Determination of isosorbide dinitrate bi

Determination of isosorbide dinitrate biotransformation in various tissues of the rabbit by capillary column gas-liquid chromatography

✍ G. S. Tam; K. Nakatsu; J. F. Brien; G. S. Marks 📂 Article 📅 1987 🏛 John Wiley and Sons 🌐 English ⚖ 454 KB 👁 1 views

A selective and sensitive capillary column gas-liquid chromatographic procedure has been developed for the simultaneous determination of isosorbide dinitrate (ISDN) and its mononitrate metabolites in rabbit blood and tissue homogenates. The method has a limit of detection of O.lng ml-l for ISDN, l n

Simultaneous determination of glyceryl t

Simultaneous determination of glyceryl trinitrate and its metabolites in plasma using capillary gas chromatography with on-column injection

✍ Langseth-Manrique, K. ;Bredesen, J. E. ;Greibrokk, T. 📂 Article 📅 1986 🏛 John Wiley and Sons 🌐 English ⚖ 442 KB 👁 2 views

A specific, sensitive, and precise assay for simultaneous quantitation of glyceryl trinitrate (GTN) and its metabolites 1,2and 1,3-glyceryl dinitrate (GDN) has been developed. The method involves one single extraction step with benzene, followed byevaporation at 55'C.One plof theextractwasinjected o

Automated determination of nifedipine in

Automated determination of nifedipine in human plasma by capillary gas chromatography with electron capture detection

✍ Lutz, D. ;Ilias, E. ;Jaeger, H. 📂 Article 📅 1986 🏛 John Wiley and Sons 🌐 English ⚖ 265 KB 👁 1 views

A rapid, sensitive, and reliable method for the determination of nifedipine in human plasma is described. Using a single-step solvent extraction and capillary gas chromatography combined with electron capturedetection, an assaysensitivityof 2 ng/ml is achieved routinely using 0.5 ml of plasma. Intac