Implementation of ultraviolet solid-phase spectrophotometry by use of derivative techniques

✍ Scribed by L.Fermín Capitán-Vallvey; Ignacio de Orbe; M.Carmen Valencia; Juan J. Berzas-Nevado

• Publisher: Elsevier Science
• Year: 1993
• Tongue: English
• Weight: 531 KB
• Volume: 282
• Category: Article
• ISSN: 0003-2670
• DOI: 10.1016/0003-2670(93)80353-m

✦ Synopsis

A spectrophotometric method for the simultaneous determination of sulpbathiaxole and sulphametaxine based on fourth-derivative solid-phase spectrophotometry is proposed. Both sulphonamides were fixed on a dextran-type cation-exchange gel at pH 2.5. The absorbance on the gel, packed in a l-mm silica cell, was measured directly. The method does not require any separation step. The range of application is between 0.20 and 12.00 pg ml-' for sulphathiaxole and between 0.20 and 15.00 pg ml-' for sulphametaxine. The acuracy and precision of the method are reported. The method was applied for the determination of sulpha thiaxole and sulphametazine in synthetic mixtures and pharmaceuticals.