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Determination of acetoacetate in urine by solid-phase spectrophotometry

✍ Scribed by F. Molina; M.C. Valencia; J. De La Torre; L.F. Capitán-Vallvey

Publisher
Elsevier Science
Year
1989
Tongue
English
Weight
523 KB
Volume
7
Category
Article
ISSN
0731-7085

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✦ Synopsis

A method for the determination of acetoacetate has been developed based on solid-phase spectrophotometry (SPS). The acetoacetate reacts with nitroprusside and glycine and the reaction product is sorbed on Dowex 1-X8 resin. The absorbance of the resin phase at 590 and 720 nm is measured directly. The calibration graph is linear up to 3.3 mg l-1 and the RSD is 1.9%. The detection limit is 7.6 micrograms l-1. The method has been applied to the determination of acetoacetate in normal and diabetic subjects' urine without pretreatment of the samples, and the results compared with those of 1H-NMR and homogeneous nitroprusside methods.

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