A method for the determination of acetoacetate has been developed based on solid-phase spectrophotometry (SPS). The acetoacetate reacts with nitroprusside and glycine and the reaction product is sorbed on Dowex 1-X8 resin. The absorbance of the resin phase at 590 and 720 nm is measured directly. The
Determination of colorant matters mixtures in foods by solid-phase spectrophotometry
✍ Scribed by F. Capitán; L.F. Capitán-Vallvey; M.D. Fernández; I. de Orbe; R. Avidad
- Publisher
- Elsevier Science
- Year
- 1996
- Tongue
- English
- Weight
- 644 KB
- Volume
- 331
- Category
- Article
- ISSN
- 0003-2670
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✦ Synopsis
A new, simple, sensitive and inexpensive method for simultaneous determination of Sunset Yellow FCF (SY), Quinoline Yellow and their respective unsulphonated products (Sudan I (SUD) and Quinoline Yellow Spirit Soluble (QYSS)) in mixtures is proposed. Sunset Yellow and Quinoline Yellow were selectively fixed in Sephadex DEAE A-25 gel (pH 5.0) and SUD and QYSS were selectively fixed in Cl8 silica gel (pH 5.0) and the absorbances of both systems were measured directly in solidphase. The applicable concentration ranges were from 3.0 to 500.0ngml-' for Quinoline Yellow Water Soluble (QYWS), from 3.0 to 200.0 ng ml-' for QYSS, from 15.0 to 500.0ng ml-' for SY and from 20.0 to 200.0 ng ml-' for SUD. The method was applied to the determination of these compounds in soft drinks, fruit liqueurs and ice creams.
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