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Immunological characterization of metallothioneins in mouse LMTK cells and in a variant resistant to cadmium

✍ Scribed by Indu Maiti; Majambu Mbikay; Claude Marengo; Jean-Paul Thirion

Publisher
John Wiley and Sons
Year
1982
Tongue
English
Weight
716 KB
Volume
112
Category
Article
ISSN
0021-9541

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✦ Synopsis

Abstract

A cadmium‐resistant variant isolated from mouse fibroblast LMTK cells can grow in the presence of 40 μM Cd^2+^. This variant retained its properties in the absence of selecting agent. Induction of metallothionein was measured in cell extracts by radioimmunoassay. The maximum amount of metallothioneins in the cells was reached after 36 hours. The cadmium resistant variant produced two times more metallothionein than the wild‐type when exposed to 10–20 μM Cd^2+^. By Ouchterlony double diffusion, the metallothioneins from cultured cells formed a line of parital identity with the mouse liver serotype and a line of complete identity with one of the two mouse kidney serotypes. These observations raise the possibility of a tissue‐specific expression of metallothionein genes.

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