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Immunoelectronmicroscopy of metaphase chromosomes

โœ Scribed by F. T. Bosman; P. K. Nakane

Publisher
Springer
Year
1982
Tongue
English
Weight
1009 KB
Volume
74
Category
Article
ISSN
1432-119X

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โœฆ Synopsis

A method for the preparation of ultrathin sections of metaphase chromosomes is described. This method was applied to human metaphase chromosomes, which were immunocytochemically stained with anti-DNA and anti-ribonucleoprotein antibodies, derived from patients with auto-immune disease. Conventionally prepared metaphase spreads as well as cytocentrifuge preparations of chromosome suspensions were studied. The results indicate that the ultrastructure of chromosomes and the immunoreactivity of chromosomal constituents are influenced by the applied preparation methods. In comparison with whole mount preparations, ultrathin sections of immunostained chromosomes allow higher resolution and more precise localization of immunoreactive sites within the chromosomal structure.

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