Banding and spiralization of human metaphase chromosomes
β Scribed by Ranjan Y. Goradia; Brian K. Davis
- Publisher
- Springer
- Year
- 1977
- Tongue
- English
- Weight
- 320 KB
- Volume
- 36
- Category
- Article
- ISSN
- 0340-6717
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β¦ Synopsis
A new technique is described which produces spiralization of human metaphase chromosomes. The important feature is heat followed by trypsin treatment. By varying conditions, it is possible to produce bands, spirals and intermediate stages. This provides a new approach to the understanding of banding and chromosome structure.
π SIMILAR VOLUMES
This paper describes a study of the role of certain cations in the alkaline pretreatment step used in the CT technique for chromosome band formation. Treatment of human chromosomes with ammonium bases or with the hydroxides of the monovalent alkali metals Na, K, or Li resulted in their rapid disinte