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Responses of mammalian metaphase chromosomes to endonuclease digestion

✍ Scribed by C. G. Sahasrabuddhe; S. Pathak; T. C. Hsu

Publisher: Springer
Year: 1978
Tongue: English
Weight: 958 KB
Volume: 69
Category: Article
ISSN: 0009-5915
DOI: 10.1007/bf00332136

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✦ Synopsis

Digestion of fixed metaphase chromosomes by endonucleases (micrococcal nuclease and DNase II) under optimal digestion conditions followed by Giemsa staining produces sharp banding patterns identical to G-bands. In 3H-thymidine labeled, synchronized metaphase cells of the chinese hamster (CHO line), the band induction is accompanied by the removal of DNA. The single strand specific nuclease S1 and DNase I do not produce such banding patterns.

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