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Immunocytochemical localization of acrosin in boar spermatozoa

✍ Scribed by Castellani-Ceresa, Lucia ;Berruti, Giovanna ;Colombo, Roberto

Publisher
John Wiley and Sons
Year
1983
Tongue
English
Weight
739 KB
Volume
227
Category
Article
ISSN
0022-104X

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✦ Synopsis

Acrosin was localized on the acrosomal membranes of boar sperm by immunofluorescence and immunoelectron microscopy. Indirect labeling of sperm head sections was performed with an Imposil (iron-dextran) antibody conjugate. The results of electron microscopy indicated a preferential deposition of the antiacrosin antibodies on the outer acrosomal membrane, although a lesser deposition was also found on the inner acrosomal membrane. No labeling was detected in the acrosomal content when such material was still present.

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