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Ca+ + localization in boar spermatozoa by the pyroantimonate technique and X-ray microanalysis

✍ Scribed by Berruti, Giovanna ;Franchi, Emilia ;Camatini, Marina

Publisher
John Wiley and Sons
Year
1986
Tongue
English
Weight
536 KB
Volume
237
Category
Article
ISSN
0022-104X

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✦ Synopsis

Intracellular, loosely bound Ca+ + has been localized electron microscopically in freshly ejaculated boar spermatozoa by in situ precipitation with potassium antimonate. Ca++ was identified as the cation precipitated by testing the EGTA-sensitivity of the precipitates and by X-ray microprobe analysis. The data obtained revealed that the outer acrosomal membrane is the preferential site for Ca+ + precipitation in the sperm head.

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