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Immunoblotting of Human Red Cell Membranes: Detection of Glycophorin B with Anti-S and Anti-s Antibodies

โœ Scribed by G. Khalid; C.A. Green


Book ID
115109603
Publisher
John Wiley and Sons
Year
1990
Tongue
English
Weight
713 KB
Volume
59
Category
Article
ISSN
0042-9007

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A simple method for purification of anti
โœ Katsumata, Y. ;Sato, M. ๐Ÿ“‚ Article ๐Ÿ“… 1985 ๐Ÿ› Springer-Verlag ๐ŸŒ English โš– 209 KB

A simple method for purification of anti-A and anti-B antibodies using glutaraldehyde-fixed human erythrocytes is described. Specific antibodies were first absorbed with the corresponding cells, then eluted by heating at 53ยฐ-55ยฐC for 15 min. The method is simple and highly efficient with a fair reco