𝔖 Bobbio Scriptorium
✦   LIBER   ✦

Immunoblot detection of antibodies to Toscana virus

✍ Scribed by Schwarz, Tino F.; Gilch, Sabine; Pauli, Christof; Jäger, Gundula

Book ID
102646536
Publisher
John Wiley and Sons
Year
1996
Tongue
English
Weight
524 KB
Volume
49
Category
Article
ISSN
0146-6615

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✦ Synopsis

Sera from patients with sandfly fever caused by Toscana virus (TOSV) infection were tested by immunoblot for specific antibody response to TOSV derived from infected Vero-E6 cells. The 28 kDa TOSV nucleoprotein (N) was identified as the major immunodominant protein recognized by immunoblot. In sera of patients with acute TOSV infection, specific antibodies of the IgM, IgA, and IgG class were detected. Using sandfly fever virus, serotypes Sicilian (SFSV) and Naples (SFNV), as antigens for immunoblot, TOSV antibody-positive sera cross-reacted with the corresponding N proteins. These sera reacted for IgM and IgG by SFSV immunoblot, and for IgM by SFNV immunoblot. The diagnosis of sandfly fever may be confirmed by TOSV immunoblot.

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