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Enhanced detection of antibodies to hepatitis C virus by use of a third-generation recombinant immunoblot assay

✍ Scribed by C. Buffet; N. Charnaux; P. Laurent-Puig; S. Chopineau; J. P. Quichon; M. J. Briantais; E. Dussaix

Publisher: John Wiley and Sons
Year: 1994
Tongue: English
Weight: 235 KB
Volume: 43
Category: Article
ISSN: 0146-6615
DOI: 10.1002/jmv.1890430312

No coin nor oath required. For personal study only.

✦ Synopsis

Abstract

Fifty‐seven sera with indeterminate results by the second generation RIBA (RIBA 2) for confirmation of hepatitis C virus (HCV) enzyme linked immunoassay (ELISA) reactivity were tested by the new third generation RIBA (RIBA 3). Thirty three (57.9%) displayed reactivity for at least one other band and were therefore classified as positive; two became negative and 22 (38%) remained indeterminate. The incidence of HCV viremia, as determined by the RNA polymerase chain reaction (PCR), was 75% for the latter sera. The data show that it is important to subject RIBA 3 indeterminate samples to PCR. © 1994 Wiley‐Liss, Inc.

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