✦ LIBER ✦

Detection of hepatitis C virus infection with recombinant immunoblot assay, synthetic immunoblot assay, and polymerase chain reaction

✍ Scribed by R. K. Chaudhary; A. Andonov; C. Maclean

Publisher: John Wiley and Sons
Year: 1993
Tongue: English
Weight: 313 KB
Volume: 7
Category: Article
ISSN: 0887-8013
DOI: 10.1002/jcla.1860070306

No coin nor oath required. For personal study only.

✦ Synopsis

The newly developed immunoblot assay, RlBA SIA (recombinant and synthetic polypeptide immunoblot assay), Chiron, Calif., was compared with the commercially available second generation recombinant immunoblot assay (RIBA-2) for the detection of antibody to hepatitis C virus (anti-HCV). The two immunoblot tests were also compared with the polymerase chain reaction (PCR) for the detection of HCV RNA.

Ninety-one percent of samples reactive by RIBA-2 were positive for anti-HCV by RlBA SIA. A total of 31 % of RIBA-2 indeterminate samples became reactive by RlBA SIA, 24% became non-reactive, and 45% remained the same.

Samples reactive by RIBA-2 or SIA from different risk groups, were mostly positive (67-1 00%) by PCR for HCV RNA. All indeterminate samples from hemophiliacs and intravenous drug users were PCR positive. RlBA SIA is more sensitive and specific than RIBA-2 and correlates well with PCR results C D 1993 Wiley-Liss. Inc.

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