✦ LIBER ✦

Improved immunoblotting for the detection and quantitative analysis of antibodies to human immunodeficiency virus

✍ Scribed by P. Portincasa; G. Conti; M. Gerloni; C. Chezzi

Publisher: Springer
Year: 1991
Tongue: English
Weight: 519 KB
Volume: 7
Category: Article
ISSN: 0393-2990
DOI: 10.1007/bf00218688

No coin nor oath required. For personal study only.

✦ Synopsis

Detection of antibodies to single HIV-1 proteins was performed by modified Western-blotting procedure. The use of [35S]-Streptavidin instead of Avidin-peroxidase conjugate greatly enhances the sensitivity of the method and enables useful quantitative analysis of results.

📜 SIMILAR VOLUMES

Immunoblotting analysis of IgA and IgM a

Immunoblotting analysis of IgA and IgM antibody to human immunodeficiency virus type 1 (HIV-1) polypeptides in seropositive infants

✍ M. C. Re; G. Furlini; M. Vignoli; G. Zauli; P. Dallacasa; M. Masi; M. La Placa 📂 Article 📅 1992 🏛 Springer 🌐 English ⚖ 549 KB

Evaluation of a line immunoassay for the

Evaluation of a line immunoassay for the differential detection of antibodies to human immunodeficiency virus

✍ R. Vranckx 📂 Article 📅 1990 🏛 Springer 🌐 English ⚖ 427 KB

Streptococcal protein G: A sensitive too

Streptococcal protein G: A sensitive tool for detection of antibodies to human immunodeficiency virus proteins in Western blot analysis

✍ Björck, L. ;Blomberg, J. 📂 Article 📅 1987 🏛 Springer-Verlag ⚖ 319 KB

Blocking-ELISA for detection of specific

Blocking-ELISA for detection of specific antibodies to the glycoproteins of the human immunodeficiency virus type 1 (HIV-1)

✍ J. C. Tardy; J. Basson; A. Douglas; M. Aymard 📂 Article 📅 1990 🏛 John Wiley and Sons 🌐 English ⚖ 403 KB 👁 1 views

## Abstract A blocking ELISA was developed to confirm the specificity of screening tests for anti‐HIV‐1 antibodies. A murine monoclonal antibody (McAb) raised against recombinant gp 160 was used in combination with a commercial technique (ELAVIA‐1). After determining the optimal experimental condit

Comparison of enzyme immunoassays and an

Comparison of enzyme immunoassays and an immunofluorescence test for detection of antibody to human immunodeficiency virus in African sera

✍ Vercauteren, G. ;van der Groen, G. ;Piot, P. 📂 Article 📅 1987 🏛 Springer-Verlag ⚖ 343 KB

Detection of seminal antibodies to human

Detection of seminal antibodies to human immunodeficiency virus in vaginal secretions after sexual intercourse: Possible means of preventing the risk of human immunodeficiency virus transmission in a rape victim

✍ Laurent Bélec; Christopher Payan; Jacques Pillot; Laurent Bélec; Mathieu Matta; 📂 Article 📅 1995 🏛 John Wiley and Sons 🌐 English ⚖ 420 KB

Detection of semen anti-human immunodeficiency virus (HIV) antibodies within the cervicovaginal secretions from a non-HIV-infected woman who has had a recent sexual intercourse with an HIV-infected man is theoretically possible since the seminal fluid from all HIV-infected men contains a high titer