Evaluation of a line immunoassay for the differential detection of antibodies to human immunodeficiency virus
β Scribed by R. Vranckx
- Publisher
- Springer
- Year
- 1990
- Tongue
- English
- Weight
- 427 KB
- Volume
- 9
- Category
- Article
- ISSN
- 0934-9723
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Detection of antibodies to single HIV-1 proteins was performed by modified Western-blotting procedure. The use of [35S]-Streptavidin instead of Avidin-peroxidase conjugate greatly enhances the sensitivity of the method and enables useful quantitative analysis of results.
Serology has been a fundamental tool to prevent post-transfusional infection with human immunodeficiency virus (HIV) and for epidemiological surveys, the first step to attempt control of the pandemia. Enzyme immunoassay is in widespread use. Nevertheless, simpler methods are needed in many countries
## Abstract A blocking ELISA was developed to confirm the specificity of screening tests for antiβHIVβ1 antibodies. A murine monoclonal antibody (McAb) raised against recombinant gp 160 was used in combination with a commercial technique (ELAVIAβ1). After determining the optimal experimental condit