Immobilized enzyme-based flowing-stream analyzer for measurement of penicillin in fermentation broths
โ Scribed by Rusling, J. F.; Luttrell, G. H.; Cullen, L. F.; Papariello, G. J.
- Book ID
- 126322966
- Publisher
- American Chemical Society
- Year
- 1976
- Tongue
- English
- Weight
- 682 KB
- Volume
- 48
- Category
- Article
- ISSN
- 0003-2700
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๐ SIMILAR VOLUMES
Three different flow-mjectlon analysis systems for measurmg pemcdhns m complex fermentation media were tested a molybdenum blue, an mdlmetnc and a potentlometnc method All the methods are based on enzymatic hydrolysis of the pemcdhn to the corresponding pemcdlolc acid using /3-lactamase, followed by
An enzyme lmmoblllzatlon techmque to measure pemclllm umcentratlon was developed Penrcdlmase IS Immoblhzed by cross-lmkmg with a very fine film of glutaraldehyde, which IS sprayed on to the sensltlve ends of two different pH glass electrodes An extremely short response time of less than 10 s IS guar