Flow-injection analysis for the measurement of penicillin V in fermentation media

✍ Scribed by Morten Carlsen; Lars H. Christensen; Jens Nielsen

• Publisher: Elsevier Science
• Year: 1993
• Tongue: English
• Weight: 667 KB
• Volume: 274
• Category: Article
• ISSN: 0003-2670
• DOI: 10.1016/0003-2670(93)80611-n

✦ Synopsis

Three different flow-mjectlon analysis systems for measurmg pemcdhns m complex fermentation media were tested a molybdenum blue, an mdlmetnc and a potentlometnc method All the methods are based on enzymatic hydrolysis of the pemcdhn to the corresponding pemcdlolc acid using /3-lactamase, followed by detection of pemcdlolc acid The &lactamase 1s unmobdlzed m a packed-bed reactor m which there 1s 100% conversion of pemcdhn to penslllolc acid, and all the methods therefore measure the sum of pemcdlolc acid and pemcdhn m the sample The lodnnetnc method was found to be the best method for measurement m fermentation media By replacing the enzyme reactor with an enzyme-free dummy reactor, it IS possible to measure the concentration of pemcdlolc acid alone, and thence the pemcdhn concentration can be determined by subtraction of the pemcdlolc acid concentration from the total response m the analyser with the enzyme reactor inserted The apphcatlon of the analyser was tested by momtormg a fed-batch penmlhn fermentation and the correlation between measurements by the FIA system and a liquid chromatographlc method was very good