𝔖 Bobbio Scriptorium
✦   LIBER   ✦

Immobilization of urea cycle enzymes. II. Characterization of immobilized argininosuccinate synthetase

✍ Scribed by Miura, Yoshiharu ;Miyamoto, Kazuhisa ;Urabe, Hideaki ;Nagata, Chikako ;Hane, Yumiko

Publisher
John Wiley and Sons
Year
1977
Tongue
English
Weight
507 KB
Volume
11
Category
Article
ISSN
0021-9304

No coin nor oath required. For personal study only.

✦ Synopsis

Abstract

Argininosuccinate synthetase (EC 6.3.4.5) was immobilized on CNBr‐activated Sepharose 4B. Properties of the immobilized enzyme are described and compared with those of the native enzyme. The immobilized enzyme was much more stable than the native enzyme at 37Β°C. It was further stabilized in the presence of the assay reagents. The optimum pH of the immobilized enzyme shifted towards alkalinity (approximately 0.5 unit). The apparent Michaelis constants measured for the immobilized enzyme were not greatly different from those measured for the native enzyme.

Urea formation from citrulline was confirmed in a continuous column reactor by the coimmobilized argininosuccinate synthetase, argininosuccinate lyase (EC 4.3.2.1), and arginase (EC 3.5.3.1).

πŸ“œ SIMILAR VOLUMES

Immobilization of urea cycle enzymes. I.
Immobilization of urea cycle enzymes. I. Characterization of immobilized carbamoylphosphate synthetase and ornithine carbamoyltransferase
✍ Miura, Yoshiharu ;Miyamoto, Kazuhisa ;Raze, J. M. ;Urabe, Hideaki πŸ“‚ Article πŸ“… 1977 πŸ› John Wiley and Sons 🌐 English βš– 453 KB

## Abstract Carbamoylphosphate synthetase (EC 2.7.2.5) and ornithine carbamoyl‐transferase (EC 2.1.3.3) extracted from frog liver were successfully immobilized on CNBr‐activated Sepharose 4B. The immobilized preparation had a better stability towards heat. The apparent Michaelis constant values for

Characterization of sand as a support fo
Characterization of sand as a support for immobilized enzymes
✍ Jeffrey E. Brotherton; Alden Emery; Victor W. Rodwell πŸ“‚ Article πŸ“… 1976 πŸ› John Wiley and Sons 🌐 English βš– 807 KB

## Abstract The potential of sand as a support for immobilized enzymes was investigated by preparing alkylamine sand and devising methods to measure the total number of amine groups present and the fraction available for immobilization of enzymes. Alcohol dehydrogenase (alcohol:NAD oxidoreductase,