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Immobilization of urea cycle enzymes. I. Characterization of immobilized carbamoylphosphate synthetase and ornithine carbamoyltransferase

✍ Scribed by Miura, Yoshiharu ;Miyamoto, Kazuhisa ;Raze, J. M. ;Urabe, Hideaki

Publisher: John Wiley and Sons
Year: 1977
Tongue: English
Weight: 453 KB
Volume: 11
Category: Article
ISSN: 0021-9304
DOI: 10.1002/jbm.820110509

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✦ Synopsis

Abstract

Carbamoylphosphate synthetase (EC 2.7.2.5) and ornithine carbamoyl‐transferase (EC 2.1.3.3) extracted from frog liver were successfully immobilized on CNBr‐activated Sepharose 4B. The immobilized preparation had a better stability towards heat. The apparent Michaelis constant values for N‐acetyl‐glutamate, ammonia, and ATP were not significantly changed by immobilization.

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