Immobilization of permeabilized whole cell penicillin
β Scribed by Shiwei Cheng; Dongzhi Wei; Qingxun Song; Xiangguo Zhao
- Publisher
- Springer Netherlands
- Year
- 2006
- Tongue
- English
- Weight
- 164 KB
- Volume
- 28
- Category
- Article
- ISSN
- 0141-5492
No coin nor oath required. For personal study only.
π SIMILAR VOLUMES
## Abstract __Penicillium chrysogenum__ was immobilized in polyacrylamide gel prepared from 5% acrylamide monomers (85% acrylamide and 15% __N__,__N__β²βmethylene bisacrylamide). Penicillin produced from glucose by the immobilized mycelium was 17% of that produced by washed mycelium. However, the ac
Escherichia coli cells with penicillin acylase activity were permeabilized with aqueous solutions of the cationic detergent N-cetyl-N,N,N-trimethylammonium bromide (CTAB), at pH 8.0 and the activity was found to have almost doubled. The concentration of CTAB, the time and temperature of treatment we