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Enhanced stability of enzymes in permeabilized and immobilized cells

✍ Scribed by H. R. Felix; K. Mosbach


Publisher
Springer Netherlands
Year
1982
Tongue
English
Weight
356 KB
Volume
4
Category
Article
ISSN
0141-5492

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B-Galactosidase was immobilized in a crosslinked poly(N-isopropylacrylamide-coacrylamide) hydrogel which exhibits an LCST(lower critical solution temperature) behavior. The hydrogel collapses above the LCST, and expands below the LCST. The temperature-dependent phase transition was around 37OC. The