Yeast cells were permeabilized by incubation in 0.8 M sorbitol followed by suspension in dilute buffer. A preincubation with 2-mercaptoethanol was also included for optimal permeabilization. More than 90% of the treated cells were stainable with methylene blue. Determinations of cell wall-synthesizi
In Situ Assays of Fungal Enzymes in Cells Permeabilized by Osmotic Shock
✍ Scribed by Sergej Šesták; Vladimı́r Farkaš
- Publisher
- Elsevier Science
- Year
- 2001
- Tongue
- English
- Weight
- 80 KB
- Volume
- 292
- Category
- Article
- ISSN
- 0003-2697
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