The structural characteristics of regenerated silk fibroin were determined IR spectra. Without ethanol treatment, the membrane has the structure of silk I. When treated with ethanol, the membrane possesses a mixed structure of silk I and silk II. The structures of the blend membrane were identified
Immobilization of glucose oxidase with the blend of regenerated silk fibroin and poly(vinyl alcohol) and its application to a 1,1′- dimethylferrocene-mediating glucose sensor
✍ Scribed by Yongcheng Liu; Xin Chen; Jianghong Qian; Haiying Liu; Zhengzhong Shao; Jiaqi Deng; Tongyin Yu
- Publisher
- Springer-Verlag
- Year
- 1997
- Tongue
- English
- Weight
- 960 KB
- Volume
- 62
- Category
- Article
- ISSN
- 0273-2289
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Before or after the blend membrane of regenerated silk fibroin (RSF), poly(viny1 alcohol) (PVA), and peroxidase is treated with ethanol, RSF, PVA, and peroxidase maintain their own structures. The conformational transition of RSF in the blend membrane is accomplished from the silk I structure to the
The structural changes of the regenerated silk fibroin membranes for immobilizing glucose oxidase were characterized with FT-IR spectra. The results of electronic absorption spectra and scanning electron microscopy illustrated that the glucose oxidase in the membranes existed in aggretates. The blen