The structural changes of the regenerated silk fibroin membranes for immobilizing glucose oxidase were characterized with FT-IR spectra. The results of electronic absorption spectra and scanning electron microscopy illustrated that the glucose oxidase in the membranes existed in aggretates. The blen
Immobilization of glucose oxidase in the composite membrane of regenerated silk fibroin and poly(vinyl alcohol): application to an amperometric glucose sensor
β Scribed by Haiying Liu; Jianghong Qian; Yongcheng Liu; Tongyin Yu; Jiaqi Deng
- Publisher
- Elsevier Science
- Year
- 1996
- Tongue
- English
- Weight
- 456 KB
- Volume
- 39
- Category
- Article
- ISSN
- 1567-5394
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A new composite membrane of poly(viny1 alcohol) (PVA) and regenerated silk fibroin (RSF) was successfully employed to immobilize horseradish peroxidase (HRP) and infrared (IR) was used to get insight in the structure of the composite membrane. An amperometric HRP-based sensor highly sensitive to hyd
Before or after the blend membrane of regenerated silk fibroin (RSF), poly(viny1 alcohol) (PVA), and peroxidase is treated with ethanol, RSF, PVA, and peroxidase maintain their own structures. The conformational transition of RSF in the blend membrane is accomplished from the silk I structure to the
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