Feature of Entrapment of Glucose Oxidase in Regenerated Silk Fibroin Membranes and Fabrication of a 1,1′-Dimethylferrocene-Mediating Glucose Sensor

✍ Scribed by Haiying Liu; Yongcheng Liu; Jianghong Qian; Tongyin Yu; Jiaqi Deng

• Publisher: Elsevier Science
• Year: 1996
• Tongue: English
• Weight: 407 KB
• Volume: 53
• Category: Article
• ISSN: 0026-265X
• DOI: 10.1006/mchj.1996.0035

✦ Synopsis

The structural characteristics of regenerated silk fibroin were determined IR spectra. Without ethanol treatment, the membrane has the structure of silk I. When treated with ethanol, the membrane possesses a mixed structure of silk I and silk II. The structures of the blend membrane were identified as a composite of the absorption bands characteristic of both molecules. SEM showed that the blend membrane has an island-sea structure and nonuniform distribution of glucose oxidase inside the membrane may exist. An amperometric glucose sensor was constructed by utilizing 1,1Јdimethylferrocene as electron transfer mediator. The effects of various experimental parameters such as pH, temperature, buffer concentration, applied potential, and scan rate are explored for optimum analytical performance. The glucose sensor responds rapidly to glucose, with steady-state current responses achieved in less than 50 s.1996 Academic Press, Inc.