Immobilization of cytochrome P-450 and electrochemical control of its activity

✍ Scribed by Nobuhiro Sugihara; Yoshiro Ogoma; Koji Abe; Yoshiyuki Kondo; Toshihiro Akaike

• Book ID: 101291554
• Publisher: John Wiley and Sons
• Year: 1998
• Tongue: English
• Weight: 353 KB
• Volume: 9
• Category: Article
• ISSN: 1042-7147
• DOI: 10.1002/(sici)1099-1581(199805)9:5<307::aid-pat788>3.0.co;2-1

✦ Synopsis

P-450 cam (camphor-induced cytochrome P-450) was immobilized on an indium tin oxide (ITO) electrode by polypyrrole and its activity was controlled electrochemically. The results showed that P-450 cam was immobilized on the ITO electrode without denaturing and the amount of P-450 cam could be easily controlled. When, the electric potential was swept repeatedly between 0.4 and 0 V, the remarkable decrease of oxygen in the reaction mixture solution was observed only in the presence of camphor. In addition, hydroxycamphor was detected only in the same system by means of gas chromatography/mass spectroscopy. These results suggested that immobilized P-450 cam catalyzed the hydroxylation of camphor by the supply of electron from the electrode. The effect of pH and ionic strength on the activity was examined, and it was found that the high activity expressed at the pH of 6.0 ± 7.0 and KCl concentration of 0.1 ± 0.2 M. The paper is the first report that P-450 enzyme activity could be controlled artificially.