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Immobilization and activity assay of cytochrome P450 on patterned lipid membranes

✍ Scribed by Yoshihiro Ueda; Kenichi Morigaki; Yoshiro Tatsu; Noboru Yumoto; Hiromasa Imaishi

Book ID
116294508
Publisher
Elsevier Science
Year
2007
Tongue
English
Weight
371 KB
Volume
355
Category
Article
ISSN
0006-291X

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P-450 cam (camphor-induced cytochrome P-450) was immobilized on an indium tin oxide (ITO) electrode by polypyrrole and its activity was controlled electrochemically. The results showed that P-450 cam was immobilized on the ITO electrode without denaturing and the amount of P-450 cam could be easily