Electrochemical reduction of cytochromes P450 using electrodes containing immobilized substrates
β Scribed by V. V. Shumyantseva; T. V. Bulko; G. P. Kuznetsova; N. F. Samenkova; T. Bachman; H. Schulze; R. D. Schmid; S. A. Usanov; A. I. Archakov
- Book ID
- 111471990
- Publisher
- Pleiades Publishing
- Year
- 2007
- Tongue
- English
- Weight
- 162 KB
- Volume
- 1
- Category
- Article
- ISSN
- 1990-7508
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The substrate specificity of a camphor-induced cytochrome P-450 (P-450 cam ) was measured by using a new assay system: electrochemical control of P-450 cam activity by protein immobilization on an electrode. Immobilized P-450 cam showed the obvious substrate specificity for hydroxylation of the subs
P-450 cam (camphor-induced cytochrome P-450) was immobilized on an indium tin oxide (ITO) electrode by polypyrrole and its activity was controlled electrochemically. The results showed that P-450 cam was immobilized on the ITO electrode without denaturing and the amount of P-450 cam could be easily