Substrate specificity of camphor-induced cytochrome P-450 Immobilized on an electrode

✍ Scribed by Nobuhiro Sugihara; Yoshiro Ogoma; Koji Abe; Yoshimasa Murakami; Yoshiyuki Kondo; Toshihiro Akaike

• Publisher: John Wiley and Sons
• Year: 1998
• Tongue: English
• Weight: 81 KB
• Volume: 9
• Category: Article
• ISSN: 1042-7147
• DOI: 10.1002/(sici)1099-1581(199812)9:12<858::aid-pat843>3.0.co;2-f

✦ Synopsis

The substrate specificity of a camphor-induced cytochrome P-450 (P-450 cam ) was measured by using a new assay system: electrochemical control of P-450 cam activity by protein immobilization on an electrode. Immobilized P-450 cam showed the obvious substrate specificity for hydroxylation of the substrate, suggesting that the simple assay system is applicable for the study of the effect of the other components of the electron transfer system on activity.