IL-8 increases integrin expression and cell motility in human chondrosarcoma cells

✍ Scribed by Chun-Yi Lee; Chun-Yin Huang; Meng-Yi Chen; Ching-Yuang Lin; Horng-Chaung Hsu; Chih-Hsin Tang

• Publisher: John Wiley and Sons
• Year: 2011
• Tongue: English
• Weight: 787 KB
• Volume: 112
• Category: Article
• ISSN: 0730-2312
• DOI: 10.1002/jcb.23179

✦ Synopsis

Abstract

Chondrosarcoma is a type of highly malignant tumor with a potent capacity to invade locally and cause distant metastasis. Chondrosarcoma shows a predilection for metastasis to the lungs. Interleukin‐8 (IL‐8), a chemokine with a defining CXC amino acid motif, is known to possess tumorigenic and proangiogenic properties. Over‐expression of IL‐8 has been detected in many human tumors. However, the effects of IL‐8 in migration and integrin expression in chondrosarcoma cells are largely unknown. In this study, we found that IL‐8 increased the migration and the expression of αvβ3 integrin in human chondrosarcoma cells. Activations of phosphatidylinositol 3‐kinase (PI3K), Akt, and AP‐1 pathways after IL‐8 treatment were demonstrated, and IL‐8‐induced expression of integrin and migration activity was inhibited by the specific inhibitor and mutant of PI3K, Akt, and AP‐1 cascades. Taken together, our results indicated that IL‐8 enhances the migration of chondrosarcoma cells by increasing αvβ3 integrin expression through the PI3K/Akt/AP‐1 signal transduction pathway. J. Cell. Biochem. 112: 2549–2557, 2011. © 2011 Wiley‐Liss, Inc.