Iduronic acid-rich proteoglycans (PGIdoA) and human post-burn scar maturation: isolation and characterization

✍ Scribed by Hari G. Garg; John W. Siebert; Arvin Garg; Peter J. Neame

• Publisher: Elsevier Science
• Year: 1995
• Tongue: English
• Weight: 502 KB
• Volume: 267
• Category: Article
• ISSN: 0008-6215
• DOI: 10.1016/0008-6215(94)00284-m

✦ Synopsis

Proteoglycans (PGs) were extracted from human hypertrophic and normal scar tissues from two different stages of maturation after bum injury, under dissociative conditions (4 M guanidinium chloride containing proteinase inhibitors). The extracts were fractionated by ion-exchange chromatography, followed by ethanol precipitation, to give PG-containing iduronic acid (PGIaoA). The size of the PGIdoA decreased with the maturation of scars. Glycosaminoglycan (GAG) chains from PGIdoA were released by alkaline borohydride treatment, and theirMrvalues were evaluated by polyacrylamide gel electrophoresis. The Mr values for PGIdoA protein cores of the hypertrophic scars (5 + years and 2-5 years) and normal scar (5 + years and 2-5 years) were 22.6, 25, 19 and 21 KDa, respectively. The iduronic acid content of PGIaoA from both types of scar increased in their maturation phase. The Mr values of PG~doA decreased with maturation. PG~doa carried the sulfate group mainly attached at C-4 of the 2-amino-2-deoxy-D-galactose residue. The NH2-terminal amino acid sequences of all the PGIdoA were similar to those of normal human skin or bone PG II (decorin) (i.e., Asp-Glu-Ala-B-Gly-lle-Gly-Pro-Glu-Val-Pro-Asp-Asp-Arg).