Inseparable iduronic acid-containing proteoglycan PG(IdoA) preparations of human skin and post-burn scar tissues: evidence for elevated levels of PG(IdoA)-I in hypertrophic scar by N-terminal sequencing
✍ Scribed by Hari G. Garg; John W. Siebert; Arvin Garg; Peter J. Neame
- Publisher
- Elsevier Science
- Year
- 1996
- Tongue
- English
- Weight
- 315 KB
- Volume
- 284
- Category
- Article
- ISSN
- 0008-6215
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✦ Synopsis
Hypertrophic scarring is characterized by disordered collagen fibrils. In order to determine whether this is, in part, a result of changes in the population of proteoglycans that are thought to be involved in regulation of collagen fibril formation, we have compared PGs from post-bum normal and hypertrophic scar tissue, as well as from human dermis and epidermis. Efforts to separate the two major iduronic acid-containing proteoglycans, decorin [PG(~doA)-II] and biglycan [PG(idog)-I], for quantitation were not successful. The different N-terminal sequences of these two iduronic acid-containing proteoglycans PG0dog)-I and -II were utilized to estimate the relative amounts in the above PG(IOoA) preparations. Normal scar, dermis and epidermis were all found to contain primarily decorin with low (< 10%) levels of biglycan relative to decorin. In contrast, iduronic acid-containing proteoglycans from hypertrophic scar were found to be approximately Abbreviations: GAG, glycosaminoglycan; PG(IdoA), iduronic acid-containing proteoglycan; PG(IdoA)-I, biglycan; PGttdoA)-II, decorin.