Identification of the C-terminally α-amidated amino acid in peptides by high-performance liquid chromatography
✍ Scribed by Wolfgang E. SCHMIDT; J. Michael CONLON; Viktor MUTT; Mats CARLQUIST; Baptist GALLWITZ; Werner CREUTZFELDT
- Book ID
- 115124905
- Publisher
- John Wiley and Sons
- Year
- 1987
- Tongue
- English
- Weight
- 554 KB
- Volume
- 162
- Category
- Article
- ISSN
- 1432-1327
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o-Phthalaldehyde in combination with N-acetyl-L-cysteine is a useful derivatization reagent for the optical resolution of enantiomeric a-H-a-amino acids, a-alkyl-a-amino acids and the corresponding acid amides. By using reversed-phase high-performance liquid chromatography with a mobile phase contai
Edited By John M. Walker. Includes Bibliographical References And Index.
A high-performance liquid chromatographic method for the enantiomeric analysis of a mixture of an a-amino acid and the corresponding acid amide is described. Reversed-phase chromatography with copper(I1) acetate and N,N-di-n-propyl-Lalanine in the mobile phase are used for the separation. For Val an
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