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Identification of the bovine brain Ins(1,4,5)P3 5-phosphatase after SDS-polyacrylamide gel electrophoresis

โœ Scribed by Manuela Lemos; Jacques E. Dumont; Christophe Erneux


Book ID
115921871
Publisher
Elsevier Science
Year
1989
Tongue
English
Weight
191 KB
Volume
249
Category
Article
ISSN
0014-5793

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Regeneration of enzyme activity after sodium dodecyl sulfate-gel electrophoresis was investigated with a purified inositol 1,4,5-trisphosphate 5-phosphatase. In order to avoid silver or Coomassie blue staining, we have used zinc acetate. This staining procedure was sensitive, rapid, and reversible p