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Regeneration of enzymatic activity after sodium dodecyl sulfate/polyacrylamide gel electrophoresis and zinc acetate staining: The example of inositol 1,4,5-trisphosphate 5-phosphatase

✍ Scribed by Anne Delvaux; Manuela Lemos; Colette Moreau; Christophe Erneux

Publisher: Elsevier Science
Year: 1990
Tongue: English
Weight: 1001 KB
Volume: 188
Category: Article
ISSN: 0003-2697
DOI: 10.1016/0003-2697(90)90555-n

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✦ Synopsis

Regeneration of enzyme activity after sodium dodecyl sulfate-gel electrophoresis was investigated with a purified inositol 1,4,5-trisphosphate 5-phosphatase. In order to avoid silver or Coomassie blue staining, we have used zinc acetate. This staining procedure was sensitive, rapid, and reversible provided that zinc cations are chelated and activity is extracted after diffusion out of the gel. The method allows some gel lane staining and identification of the enzyme based on catalytic activity.