✦ LIBER ✦

Identification of Oxidized Methionine in Peptides

✍ Scribed by Fija M. Lagerwerf; Marco van de Weert; Wigger Heerma; Johan Haverkamp

Publisher: John Wiley and Sons
Year: 1996
Tongue: English
Weight: 465 KB
Volume: 10
Category: Article
ISSN: 0951-4198
DOI: 10.1002/(sici)1097-0231(199612)10:15<1905::aid-rcm755>3.0.co;2-9

No coin nor oath required. For personal study only.

✦ Synopsis

The positive-and negative-ion collision-induced dissociation spectra of peptides containing methionine, methionine sulphoxide and methionine sulphone have been studied. Characteristic fragmentations were identified and evaluated as possible indicators for the presence of oxidized methionine residues in peptides. It was found that the elimination of CH,SOH ( -64 u) from [M+Hl+ is unique for peptides that contain methionine sulphoxide. Sequence ions containing the oxidized methionhe undergo the same elimination, allowing unambiguous sequence determination. Methionine sulphone exhibits an analogous elimination of CH,SO,H ( -80 u) from the protonated molecule, but not from sequence ions.

📜 SIMILAR VOLUMES

Identification of oxidized methionine re

Identification of oxidized methionine residues in peptides containing two methionine residues by derivatization and matrix-assisted laser desorption/ionization mass spectrometry

✍ Klaus Hollemeyer; Elmar Heinzle; Andreas Tholey 📂 Article 📅 2002 🏛 John Wiley and Sons 🌐 English ⚖ 113 KB 👁 2 views

Sequencing of oxidized methionine-contai

Sequencing of oxidized methionine-containing peptides for protein identification

✍ Wenjun Mo; Yuliang Ma; Toshifumi Takao; Thomas A. Neubert 📂 Article 📅 2000 🏛 John Wiley and Sons 🌐 English ⚖ 41 KB 👁 1 views

Analysis of peptides containing oxidized

Analysis of peptides containing oxidized methionine and/or tryptophan by fast atom bombardment mass spectrometry

✍ Renee M. Wagner; Blair A. Fraser 📂 Article 📅 1987 🏛 John Wiley and Sons 🌐 English ⚖ 261 KB 👁 2 views

Treatment of peptides containing methionine and/or tryptophan with dimethylsuifoxide/hydrochloric acidlacetic acid resulted in oxidation of these amino acids respectively to methionine sulfoxide and oxyindolalanine. This reaction was monitored by fast atom bombardment mass spectrometry using a dithi

Selective site-specific fenton oxidation

Selective site-specific fenton oxidation of methionine in model peptides: Evidence for a metal-bound oxidant

✍ Dawn R. Dufield; George S. Wilson; Richard S. Glass; Christian Schöneich 📂 Article 📅 2004 🏛 John Wiley and Sons 🌐 English ⚖ 182 KB

The metal-catalyzed oxidation (MCO) of proteins represents an important pathway for protein degradation. Although many mechanistic details of MCO are currently unknown, such mechanistic information would greatly benefit formulation scientists in the rational design and analysis of protein formulatio

Periodate oxidation of methionine in pro

Periodate oxidation of methionine in proteins

✍ R.Bryan Yamasaki; David T. Osuga; Robert E. Feeney 📂 Article 📅 1982 🏛 Elsevier Science 🌐 English ⚖ 617 KB

Treatment of free methionine with an equimolar amount of periodate gave nearly quantitative formation of the sulfoxide; treatment of free methionine sulfoxide with equimolar periodate gave nearly equal amounts of the original sulfoxide and the sulfone. Treatments of OS-1 .O% solutions of the followi

Myeloperoxidase-mediated oxidation of me

Myeloperoxidase-mediated oxidation of methionine

✍ Min-Fu Tsan 📂 Article 📅 1982 🏛 John Wiley and Sons 🌐 English ⚖ 699 KB

The rnyeloperoxidase-mediated oxidation of methionine was studied using a purified canine myeloperoxidase preparation. The system required the simultaneous presence of myeloperoxidase, H202, and a halide anion. When 0.1 mM H 2 0 2 was used, the system has a pH optimum of approximately pH 5-5.5. Brom