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Analysis of peptides containing oxidized methionine and/or tryptophan by fast atom bombardment mass spectrometry

✍ Scribed by Renee M. Wagner; Blair A. Fraser

Publisher: John Wiley and Sons
Year: 1987
Tongue: English
Weight: 261 KB
Volume: 14
Category: Article
ISSN: 1076-5174
DOI: 10.1002/bms.1200140204

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✦ Synopsis

Treatment of peptides containing methionine and/or tryptophan with dimethylsuifoxide/hydrochloric acidlacetic acid resulted in oxidation of these amino acids respectively to methionine sulfoxide and oxyindolalanine. This reaction was monitored by fast atom bombardment mass spectrometry using a dithiothreitoVdithioerythrito1 liquid matrix. Under these conditions, only methionine and tryptophan were oxidized. Comparison of mass spectra of a sample before and after oxidation should provide a rapid screening procedure for determination of these residues in peptides.

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