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Identification of Microorganisms by Mass Spectrometry (Wilkins/Identification of Microorganisms by Mass Spectrometry) || Method Reproducibility and Spectral Library Assembly for Rapid Bacterial Characterization by Metastable Atom Bombardment Pyrolysis Mass Spectrometry

โœ Scribed by Wilkins, Charles L.; Lay, Jackson O.


Publisher
John Wiley & Sons, Inc.
Year
2006
Weight
246 KB
Category
Article
ISBN
0471748641

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Identification of Microorganisms by Mass
โœ Wilkins, Charles L.; Lay, Jackson O. ๐Ÿ“‚ Article ๐Ÿ“… 2006 ๐Ÿ› John Wiley & Sons, Inc. โš– 185 KB

During the 1980s to mid 1990s pyrolysis mass spectrometry (PyMS) was extensively researched as a physico-chemical method for classifying and identifying bacteria. However, by the turn of the century few researchers were still active in this field and had cooled to this fingerprinting approach. This

Identification of Microorganisms by Mass
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This chapter will address the applications of protein-based bioinformatics to analysis of microorganisms introduced intact into the instrumental system for rapid processing and analysis. Strategies that require offline extraction and fractionation of proteins will not be discussed. Although the ampl

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โœ Wilkins, Charles L.; Lay, Jackson O. ๐Ÿ“‚ Article ๐Ÿ“… 2006 ๐Ÿ› John Wiley & Sons, Inc. โš– 60 KB

The concept of rapid microorganism identification using matrix-assisted laser desorption/ionization mass spectrometry (MALDI MS) dates back to the mid-1990s. Prior to 1998 researchers relied on visual inspection in an effort to demonstrate feasibility of MALDI MS for bacterial identification. [1][2]