The use of preparatory electrophoretic methods, such as paper curtains or starch blocks, has not been particularly successful for the fractionation of mucopolysaccharide-protein complexes. Electrophoresis of these polar materials, which form viscous solutions, suffers from the same problems of slow
Identification of acid mucopolysaccharides by micro electrophoresis
β Scribed by J.Kenneth Herd
- Publisher
- Elsevier Science
- Year
- 1968
- Tongue
- English
- Weight
- 254 KB
- Volume
- 23
- Category
- Article
- ISSN
- 0003-2697
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Gas chromatography was used for the determination of monosaccharides obt,ained b)-depolymerisation of acid mucopolysaccharides (AMPS). Purified AMPS and AMPS precipitated from the urine of patients with mucopolysaccharidoses can be identified in this manner. The utility of the technique was illustra
## Abstract The changes in mouse serum protein distribution after administration of lentinan, an antitumor polysaccharide, were examined using micro twoβdimensional electrophoresis in the absence of denaturing agents. Three protein spots were markedly increased in level and two of them were identif