Identification of a subclass of double minute chromosomes containing centromere-associated DNA

✍ Scribed by David W. Hammond; Barry W. Hancock; Dr. Malcolm H. Goyns

• Book ID: 102221906
• Publisher: John Wiley and Sons
• Year: 1994
• Tongue: English
• Weight: 470 KB
• Volume: 10
• Category: Article
• ISSN: 1045-2257
• DOI: 10.1002/gcc.2870100210

✦ Synopsis

In a study of abnormal chromosomes in non-Hodgkin's lymphoma (NHL) cells we have identified one w e which contained extrachromosomal chromatin bodies that, on the basis of their morphology and negative C-banding, appeared to be double minute chromosomes (dmin). However, fluorescence in-situ hybridization (FISH) analysis using an X-specific centromeric alphoid repeat probe and a pan-centromere probe, clearly demonstrated the presence of centromere-associated DNA in these dmin. FISH analysis with the pan-centromere probe of the dmin in neuroblastoma and sarcoma cells failed to reveal the presence of centromere-associated DNA, but analysis of two cases of acute myeloid leukaemia cells revealed centromere-associated D N A in 25% of their dmin. These data indicate the existence of dmin that contain centromere-associated D N A and suggest that such dmin might represent a new class of extrachromosomal chromatin bodies. Genes Chromosom Cancer /0:/39-/42 (1994).