Identification of a novel protein binding to hepatitis C virus core protein
β Scribed by Yun-ru Chen; Tian-yan Chen; Shu-lin Zhang; Shu-mei Lin; Ying-ren Zhao; Feng Ye; Xi Zhang; Lei Shi; Shuang-suo Dang; Min Liu
- Book ID
- 108952285
- Publisher
- John Wiley and Sons
- Year
- 2009
- Tongue
- English
- Weight
- 290 KB
- Volume
- 24
- Category
- Article
- ISSN
- 0815-9319
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Three novel monoclonal antibodies (MAbs) were established against a recombinant hepatitis C virus (HCV) core protein derived from cloned genotype 1 b HCV cDNA. MAbs C7-50 and C8-59 recognize a conserved linear epitope represented by amino acid residues 21 to 40 of the nucleocapsid protein. MAb C8-48
## Abstract The binding sites for human antibodies recognising antigenic domains within the hepatitis C virus (HCV) core protein were analyzed using synthetic peptides. Omission peptide analogues where a pair of residues was sequentially omitted were produced corresponding to the regions 1β18, 11β2
The sequence encoding the truncated core protein (amino acids 1-98) of hepatitis C virus (HCc) was expressed in E. coli for production of HCc (1-98) , or fused with the truncated core antigen (HBcAg) and segments from the preS1 and preS2 regions from hepatitis B virus (HBV) for production of HBcPreS