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Identification and cloning of a plasmid-encoded erythromycin resistance determinant from Lactobacillus reuteri

✍ Scribed by Lars T. Axelsson; Siv E.I. Ahrné; Marie C. Andersson; Sten R. Ståhl


Book ID
119078962
Publisher
Elsevier Science
Year
1988
Tongue
English
Weight
684 KB
Volume
20
Category
Article
ISSN
0147-619X

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The erythromycin resistance gene (Em(r)) from Campylobacter jejuni ABA94 plasmid DNA was cloned into the pUC18 vector and then expressed in Escherichia coli. The location of the Em(r) determinant on the chimeric plasmid was determined by restriction endonuclease mapping within a 0.8-kb EcoRI fragmen