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Cloning and expression of the erythromycin resistance determinant fromCampylobacter jejuniinEscherichia coli

โœ Scribed by R. Son; A. Ansary

Publisher
Springer
Year
1995
Tongue
English
Weight
200 KB
Volume
11
Category
Article
ISSN
1573-0972

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โœฆ Synopsis

The erythromycin resistance gene (Em(r)) from Campylobacter jejuni ABA94 plasmid DNA was cloned into the pUC18 vector and then expressed in Escherichia coli. The location of the Em(r) determinant on the chimeric plasmid was determined by restriction endonuclease mapping within a 0.8-kb EcoRI fragment. This fragment then hybridized to the 78-kb plasmid DNA but not to the 3.3-or 12.6-kb plasmid DNA of Campylobacter jejuni ABA94. Em(r) in Campylobacter jejuni is therefore probably plasmid-mediated.

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