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Cloning and expression of the sucrose phosphorylase gene fromLeuconostocmesenteroidesinEscherichia coli

✍ Scribed by Jin-Ha Lee; Young-Hwan Moon; Nahyun Kim; Young-Min Kim; Hee-Kyoung Kang; Ji-Yeon Jung; Emad Abada; Seong-Soo Kang; Doman Kim

Publisher
Springer Netherlands
Year
2007
Tongue
English
Weight
425 KB
Volume
30
Category
Article
ISSN
0141-5492

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An amylase gene was identified in a Streptococcus bovis 033 lambda gtWES lambda B genomic library. Using a starch overlay and a Congo red-iodine staining procedure, amylase positive clones could be identified by zones of clearing. Ten amylase positive clones were identified using this procedure. The