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Cloning and sequencing of a plasmid-mediated erythromycin resistance determinant from Staphylococcus xylosus

✍ Scribed by Ian D. Milton; Carey L. Hewitt; Colin R. Harwood

Book ID
109317790
Publisher
John Wiley and Sons
Year
1992
Tongue
English
Weight
335 KB
Volume
97
Category
Article
ISSN
0378-1097

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The erythromycin resistance gene (Em(r)) from Campylobacter jejuni ABA94 plasmid DNA was cloned into the pUC18 vector and then expressed in Escherichia coli. The location of the Em(r) determinant on the chimeric plasmid was determined by restriction endonuclease mapping within a 0.8-kb EcoRI fragmen