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Identification and chromosomal localisation by fluorescence in situ hybridisation of human gene of phosphoinositide-specific phospholipase C β1

✍ Scribed by Daniela Peruzzi; Giuseppe Calabrese; Irene Faenza; Lucia Manzoli; Alessandro Matteucci; Fernando Gianfrancesco; Anna Maria Billi; Liborio Stuppia; Giandomenico Palka; Lucio Cocco


Book ID
117771864
Publisher
Elsevier Science
Year
2000
Tongue
English
Weight
567 KB
Volume
1484
Category
Article
ISSN
1388-1981

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A cloned, 40 kb, genomic DNA fragment, containing the last exon of the gene for human cytochrome c oxidase subunit VIb and its flanking sequences, was used as a probe to localize the subunit VIb gene on human metaphase chromosomes. The probe was labelled with Bio-11-dUTP and detected by fluorescence